Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia

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Biological, serological and molecular typing of potato virus Y (PVY) isolates from Tunisia.

In Tunisia, potato virus Y (PVY) currently presents a significant threat to potato production, reducing tuber yield and quality. Three hundred and eighty-five potato samples (six different cultivars) collected in autumn 2007 from nine regions in Tunisia were tested for PVY infection by DAS-ELISA. The virus was detected in all regions surveyed, with an average incidence of 80.26%. Subsequently, ...

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Induction of Resistance to Potato Virus Y (PVY) Using Hairpin Construct of Coat Protein

Potato virus Y (PVY) is one of the most damaging viruses of potato plants which infecting most cultivars and causing significant yield and economical losses. The application of the concept of pathogen derived resistance opened new horizons for the development of virus-resistant plants. This research was carried out to study RNA silencing to engineered potato plants that are resistant to potato ...

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Resistance to Potato virus Y (PVY) in potato cv. Pito transformed with the P1 gene of PVY

ACADEMIC DISSERTATION IN PLANT PATHOLOGY To be presented with the permission of the Faculty of Agriculture and Forestry of the University of Helsinki for public criticism in Viikki, Auditorium B2, on June 30th, at 12 o'clock noon. HELSINKI 2000 2 Mäki-Valkama, T. Resistance to Potato virus Y (PVY) in potato cv. Pito transformed with the P1 gene of PVY. Doctoral thesis in plant pathology. 43 p. ...

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Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).

A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...

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Study of biological and molecular characterization of pepper-PVY isolated from Tehran pepper fields and it’s comparison with other PVY isolates

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

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ژورنال

عنوان ژورنال: Genetics and Molecular Research

سال: 2016

ISSN: 1676-5680

DOI: 10.4238/gmr15045165